Journal: Oncology Letters

Article Title: The effects of hemocyanin on T cells cultured in vitro

doi: 10.3892/ol.2017.7587

Figure Lengend Snippet: Cell quantity and morphology assay. (A) Quantity of T cells analyzed by cell counting. (B) T cell morphology analysis via optical microscopy (magnification, ×50). Bars represent the mean ± standard deviation (n=3). Hem, hemocyanin.

Article Snippet: T cell cytotoxicity analysis HepG2 (American Type Culture Collection, Manassas, VA, USA) were collected at logarithmic growth phase for use as target cells, and the concentration of cells was adjusted to 1×10 5 cells/ml.

Techniques: Morphology Assay, Cell Counting, Microscopy, Standard Deviation

Journal: Oncology Letters

Article Title: The effects of hemocyanin on T cells cultured in vitro

doi: 10.3892/ol.2017.7587

Figure Lengend Snippet: T cell phenotype assay by flow cytometry. (A) T cell phenotype distribution. *P<0.05. (B) The flow cytometry results of the CD3+, CD3+CD4+, CD3+CD8+ and CD4+CD25+ phenotypes. Bars represent the mean ± standard deviation (n=3). CD, cluster of differentiation; Hem, hemocyanin.

Article Snippet: T cell cytotoxicity analysis HepG2 (American Type Culture Collection, Manassas, VA, USA) were collected at logarithmic growth phase for use as target cells, and the concentration of cells was adjusted to 1×10 5 cells/ml.

Techniques: Flow Cytometry, Standard Deviation

Journal: Oncology Letters

Article Title: The effects of hemocyanin on T cells cultured in vitro

doi: 10.3892/ol.2017.7587

Figure Lengend Snippet: T cell cytotoxicity analysis via an MTT assay. Bars represent the mean ± standard deviation (n=3). *P<0.05 and **P<0.01. Hem, hemocyanin.

Article Snippet: T cell cytotoxicity analysis HepG2 (American Type Culture Collection, Manassas, VA, USA) were collected at logarithmic growth phase for use as target cells, and the concentration of cells was adjusted to 1×10 5 cells/ml.

Techniques: MTT Assay, Standard Deviation

Journal: Frontiers in Pharmacology

Article Title: The dual effect of endoplasmic reticulum stress in digestive system tumors and intervention of Chinese botanical drug extracts: a review

doi: 10.3389/fphar.2024.1339146

Figure Lengend Snippet: Chinese botanical drug extracts against digestive system tumors through ERS.

Article Snippet: , Quercetin , Styphnolobium japonicum (L.) Schott , , HepG2 cells , GRP78 Snail↓ , Inhibits tumor growth and proferliferation , .

Techniques: Migration, Mouse Assay

Journal: Nature Communications

Article Title: HNF4A and HNF1A exhibit tissue specific target gene regulation in pancreatic beta cells and hepatocytes

doi: 10.1038/s41467-024-48647-w

Figure Lengend Snippet: a Top Gene Ontology (GO) Biological Processes (BP) commonly identified in both EndoC-βH1 and human islet HNF4A ChIP-Seq. b Top GO BP identified in D35 βLC HNF4A ChIP-Seq, in side-by-side comparisons with EndoC-βH1 and human islet samples. c Top GO BP in EndoC-βH1 in comparison with HepG2 HNF4A ChIP-Seq. d Venn diagram showing overlaps in HNF4A-bound target genes in EndoC-βH1 cells, human islets, and D35 βLCs based on ChIP-Seq peaks mapping within 10 kb of the transcription start site (TSS) (number of total peaks with no filtering shown in brackets). The table provides a list of the common beta-cell target gene loci identified, of which some were also replicated in D20 EPs (in green). Analysis and visualization of GO data is based on the ChIPseeker R package (see Methods).

Article Snippet: For overexpression in EndoC-βH1 or HepG2 cells, plasmid constructs were transfected using FuGene 6 (Promega) at a ratio of 1:6 of DNA (in μg):FuGene reagent (in μl).

Techniques: ChIP-sequencing, Comparison

Journal: Nature Communications

Article Title: HNF4A and HNF1A exhibit tissue specific target gene regulation in pancreatic beta cells and hepatocytes

doi: 10.1038/s41467-024-48647-w

Figure Lengend Snippet: a Topmost common and distinct Gene Ontology (GO) biological processes (BP) of HNF4A ChIP-Seq targets in D8 hepatoblasts and HepG2 cells. Analysis and visualization of GO data is based on the ChIPseeker R package (see Methods). b Venn diagram showing overlaps in HNF4A-bound beta cell and hepatic cell target genes based on ChIP-Seq peaks within 10 kb of the transcription start site (TSS). Table provides a list of gene loci identified in both cell types. c IGV tracks showing ChIP-Seq peaks that map to the nearest genes in selected loci in hepatic cells. The scale used to visualize peaks in IGV is indicated on the right side of each track. The chromosomal location near the peak region is indicated. d Luciferase reporter analysis of CDKN2AIP , HAAO and MAP3K11 promoter activities in HepG2 cells ( n = 3 for HAAO / MAP3K11 ; n = 4 for CDKN2AIP ). Data are presented as mean ± SD. Each data point represents one independent experiment. *** indicates p < 0.001, ** indicates p < 0.01, relative to Empty si-HNF4A control in the presence of the promoter. $ indicates p < 0.05 relative to WT, based on one-way ANOVA with Tukey’s post-hoc test. Source data and exact P values are provided in the file.

Article Snippet: For overexpression in EndoC-βH1 or HepG2 cells, plasmid constructs were transfected using FuGene 6 (Promega) at a ratio of 1:6 of DNA (in μg):FuGene reagent (in μl).

Techniques: ChIP-sequencing, Luciferase, Control

Journal: Nature Communications

Article Title: HNF4A and HNF1A exhibit tissue specific target gene regulation in pancreatic beta cells and hepatocytes

doi: 10.1038/s41467-024-48647-w

Figure Lengend Snippet: a Topmost common and distinct gene ontology (GO) biological processes (BP) from HNF4A- and HNF1A-bound target genes in human islets. b Venn diagram showing overlap in HNF4A- and HNF1A-bound target genes in human islets based on ChIP-Seq peaks within 10 kb of the transcription start site (TSS) (number of total peaks with no filtering shown in brackets). Table provides a consensus list of the commonly-bound target genes. c Venn diagram showing overlaps in HNF4A- and HNF1A-bound target genes in HepG2 cells based on ChIP-Seq peaks within 10 kb of the transcription start site (TSS) (number of total peaks with no filtering shown in brackets). HNF4A-bound targets in HepG2 cells are from the consensus list of genes. d Topmost common and distinct gene ontology (GO) biological processes (BP) from HNF4A- and HNF1A-bound target genes in HepG2 cells. Analysis and visualization of GO data is based on the ChIPseeker R package (see Methods).

Article Snippet: For overexpression in EndoC-βH1 or HepG2 cells, plasmid constructs were transfected using FuGene 6 (Promega) at a ratio of 1:6 of DNA (in μg):FuGene reagent (in μl).

Techniques: ChIP-sequencing